Skip to main content
Figure 4 | Epigenetics & Chromatin

Figure 4

From: Chromatin regulated interchange between polycomb repressive complex 2 (PRC2)-Ezh2 and PRC2-Ezh1 complexes controls myogenin activation in skeletal muscle cells

Figure 4

H3K27me3/H3S28ph switch specifically influences the chromatin binding of the PRC2-Ezh2 complex but not PRC2-Ezh1. (A) Nuclear extracts from C2C12 myoblasts (growth medium (GM)) and myotubes (differentiation medium (DM), 48 h after differentiation induction) were incubated with peptides representing unmodified H3, K27me3-H3 or the double modification K27me3S28ph-H3. Binding of Ezh2, Suz12 and Eed was tested by immunoblot. (B) Nuclear extracts from C2C12 cells cultured in DM, 48 h after induction of differentiation, were incubated with the same peptides as those listed in (A) and the binding of Ezh2 and Ezh1 was tested by immunoblot.

Back to article page

Epigenetics & Chromatin

ISSN: 1756-8935