Methylation changes at differentially methylated regions (DMRs) after treatment with 5 Azacytidine. (A) Methylation levels of each loci in Sum159 (control) and after treatment with 5 Azacytidine. #1 = 1 nM treatment, #2 = 3 nM treatment. Each data point represents an individual CpG: (i) GNAS XL; (ii) KvDMR; (iii) MCTS2; (iv) MEST (g); (v) RB1; (vi) ZIM2/PEG3; (vii) ZAC; (viii) DIRAS3 (1); (ix) DIRAS3 (2); (x) ZDBF2; (xi) DIRAS3 (3); (xii) GNAS 1A; (xiii) GRB10 (s); (xiv) MEST (s); (xv) NESP55; (xvi) IGF2 (0); (xvii) IGF2 (2); (xviii) MEG3. (b) Percent reduction of methylation at each DMR analysed. Red = maternal germ-line DMRs; pink = maternal somatic DMRs; dark blue = paternal germ-line DMRs; light blue = paternal somatic DMRs. Error bars represent the range of difference between the two experiments.