MacroH2A1 is specifically incorporated into the female Tbg promoter. Chromatin immunoprecipitation (ChIP) analysis of the association of macroH2A1 with key gene promoters involved in lipid metabolism. (a) ChIP analysis of macroH2A1 enrichment level on Tbg and Lpl promoters in females. The graph shows that macroH2A1 was enriched at Tbg promoter but not at the Lpl promoter in female hepatocytes. (b) The Tbg promoter in male mice was not significantly occupied by macroH2A1. ChIP using liver chromatin from wild-type (WT) animals and H2afy-/- knockout (KO) mice was performed using anti-macroH2A1 antibody. Quantitative PCR was then performed with specific primers for promoter regions of the Tbg and Lpl genes as indicated in Materials and methods. The results are presented as the fold enrichment in wild-type animals compared with Tbg promoter DNA amplified in KO female mice. The graph represents the average of three independent experiments.