Figure 3

High-resolution analysis of chromatin domains at neocentromeres from IMS13q and CHOP13q cell lines. (a) A 650 kb genomic region surrounding the neocentromere in cell line IMS13q, showing the results for the chromatin immunoprecipitation (ChIP) analysis using antibodies to centromere protein (CENP)-A and H3K4me2. Arrows indicate small CENP-A domain colocalizing with the 5' end of genes (see Figure 5). Triangle indicates small histone H3 dimethylated at lysine 4 (H3K4me2) domain within the CENP-A domain. The H3K4me2 ChIP from CHOP13 serves as a control for IMS13q neocentromere region. H3K9me3 ChIP results for IMS13q and BBB are shown. (b) A 7 Mbp region containing the IMS13q CENP-A domain in band 13q32.1 and the H3K9me3 domain seen in this cell line in chromosome band 13q33.1. Note BBB CENP-A and H3K9me3 domain positions indicated (see Figure 2). (c) A 650 kb genomic region surrounding the neocentromere in cell line CHOP13q; ChIP for CENP-A and H3K4me2 is shown. The H3K4me2 ChIP from IMS13q serves as a control for CHOP13q neocentromere region. For domain coordinates and P values, see Table 1.