Figure 2

High-resolution analysis of chromatin domains at the BBB neocentromere. A 650 kb genomic region containing the neocentromere in cell line BBB, showing (a) the UCSC Hg18 genome coordinates from band 13q33.1 and (b) the coverage of the region on the Affymetrix tiling array, where gaps represent repetitive DNA not included on the array. (c) Results from chromatin immunoprecipitation (ChIP) with antibodies to centromere protein (CENP)-A from cell line BBB; model-based analysis of tiling-array (MAT) score. Horizontal lines indicate major and minor domains. Arrows indicate small domains that colocalize with the 5' end of the genes (see Figure 5). (d) ChIP with histone H3 dimethylated at lysine 4 (H3K4me2) antibody from cell line BBB;. Triangle indicates small domain within CENP-A domain. (e) ChIP with H3K4me2 antibody from fibroblast cell line 3B2, MAT P < 1.6 × 10-⁵, which serves as a non-neocentric control for BBB (f) The genes in the region. (g) ChIP with antibodies to CENP-C from cell line BBB. (h) ChIP with antibodies to H3K9me3 from cell line BBB. (i) ChIP with antibodies to heterochromatin protein (HP)1α from cell line BBB. (j) ChIP with antibodies to HP1γ from cell line BBB. (k) ChIP with antibodies to H3K9me3 from cell line IMS13q. (l) Repeat Masker tracks, where the 21.6 kb transposon-free region colocalizing with the H3K9me3 and HP1 domains can be seen. For domain coordinates and P values, see Table 1.