Skip to main content
Figure 5 | Epigenetics & Chromatin

Figure 5

From: Roles for common MLL/COMPASS subunits and the 19S proteasome in regulating CIITA pIV and MHC class II gene expression and promoter methylation

Figure 5

UTX recruitment correlates with a reduction in H3K27 trimethylation. (a-c) UTX associates with cytokine-inducible promoters. ChIP assays were carried out in HeLa cells stimulated with IFN-γ for 0 to 18 hours. Lysates were immunoprecipitated with control or endogenous UTX antibody. Associated DNA was isolated and analyzed via real-time PCR, using primers and probe spanning (a) CIITA pIV, (b) the MHC-II promoter or (c) the GAPDH promoter. Data are presented as percentage input. Values represent mean ± SEM of (n = 3) independent experiments. (d-f) H3K27 trimethylation was lost upon cytokine stimulation. ChIP assays were carried out in HeLa cells stimulated with IFN-γ for 0 to 18 hours. Lysates were immunoprecipitated with control or endogenous H3K27me3 antibody. Associated DNA was isolated and analyzed via real-time PCR using primers and probe spanning (d) CIITA pIV, (e) the MHC-II promoter or (f) the GAPDH promoter. Data are presented as percentage input. IgG isotype control values were 0.001 ± 0.0002 (CIITA pIV), 0.08 ± 0.02 (MHC-II promoter) and 0.001 ± 0.0009 (GAPDH promoter). Values represent mean ± SEM of (n = 3) independent experiments.

Back to article page

Epigenetics & Chromatin

ISSN: 1756-8935