Figure 4From: Roles for common MLL/COMPASS subunits and the 19S proteasome in regulating CIITA pIV and MHC class II gene expression and promoter methylationWDR5 knockdown does not affect H3K18ac. (a, b) HeLa cells transfected with scrambled control or WDR5-specific siRNA were stimulated with IFN-γ and subjected to ChIP assay. Lysates were immunoprecipitated with control or endogenous H3K18ac antibody. Associated DNA was isolated and analyzed via real-time PCR as described in Figure 2, using primers and probes specific for (a) MHC-II promoter or (b) CIITA pIV. Data are presented as percentage input. IgG Isotype control values were 0.06 ± 0.03 (MHC-II promoter) and 0.003 ± 0.002 (CIITA pIV). Values represent mean ± SEM of (n = 3) independent experiments.Back to article page