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Figure 4 | Epigenetics & Chromatin

Figure 4

From: Roles for common MLL/COMPASS subunits and the 19S proteasome in regulating CIITA pIV and MHC class II gene expression and promoter methylation

Figure 4

WDR5 knockdown does not affect H3K18ac. (a, b) HeLa cells transfected with scrambled control or WDR5-specific siRNA were stimulated with IFN-γ and subjected to ChIP assay. Lysates were immunoprecipitated with control or endogenous H3K18ac antibody. Associated DNA was isolated and analyzed via real-time PCR as described in Figure 2, using primers and probes specific for (a) MHC-II promoter or (b) CIITA pIV. Data are presented as percentage input. IgG Isotype control values were 0.06 ± 0.03 (MHC-II promoter) and 0.003 ± 0.002 (CIITA pIV). Values represent mean ± SEM of (n = 3) independent experiments.

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