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Figure 3 | Epigenetics & Chromatin

Figure 3

From: Roles for common MLL/COMPASS subunits and the 19S proteasome in regulating CIITA pIV and MHC class II gene expression and promoter methylation

Figure 3

Knockdown of a common MLL/COMPASS subunit decreases H3K4me3. (a) WDR5 knockdown efficiently reduces WDR5 expression. HeLa cells were transfected with control or WDR5-specific siRNA, harvested and subjected to western blot analysis for (top) endogenous WDR5 and (bottom) endogenous tubulin. (b-c) WDR5 knockdown decreases H3K4me3. HeLa cells transfected with scrambled control or WDR5-specific siRNA were stimulated with IFN-γ and subjected to ChIP assay. Lysates were immunoprecipitated with control or endogenous H3K4me3 antibody. Associated DNA was isolated and analyzed via real-time PCR as described in Figure 1, using primers and probes specific for (b) the MHC-II promoter or (c) CIITA pIV. Data are presented as percentage input. IgG Isotype control values were 0.06 ± 0.03 (MHC-II promoter) and 0.003 ± 0.002 (CIITA pIV). Values represent mean ± SEM of (n = 3) independent experiments. *P < 0.05 vs control siRNA.

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