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Table 1 Real time polymerase chain reaction (PCR) primers.

From: Transcription-dependent silencing of inducible convergent transgenes in transgenic mice

Real time PCR primers

h GM-CSF mRNA

CACTGCTGCTGAGATGAATGAAA

 

GTCTGTAGGCAGGTCGGCTC

m GM-CSF mRNA

ATGCCTGTCACGTTGAATGAAG

 

GCGGGTCTGCACACATGTTA

m GAPDH mRNA

TGGTGAAGCAGGCATCTGAG

 

TGTTGAAGTCGCAGGAGACAAC

ChIP primers

h GM-CSF enhancer

GGAGCCCCTGAGTCAGCAT

 

CATGACACAGGCAGGCATTC

h GM-CSF promoter

TGTCGGTTCTTGGAAAGGTTCA

 

TGTGGAATCTCCTGGCCCTTA

h GM-CSF intron 2 (+ 469 to 533 bp)

ATGGCAGTCACATGAGCTCCTT

 

TGAAGTGACCCCCACTTTACCA

m CD2 promoter

CTCTCTCCTTCCCCATCTCTACCT

 

CAACCTGAACCACGTGTCTTTC

m chromosome 1 (mChrom1)

CATAGATGAAGCTGCCACATAGGT

 

GTGGGCAAGGACAAAGCATTA

  1. GM-CSF, granulocyte-macrophage colony-stimulating factor; GAPDH, glyceraldehyde phosphate dehydrogenase
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Epigenetics & Chromatin

ISSN: 1756-8935