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Figure 5 | Epigenetics & Chromatin

Figure 5

From: Transcription-dependent silencing of inducible convergent transgenes in transgenic mice

Figure 5

Analysis of inverted repeats in granulocyte-macrophage colony-stimulating factor (GM-CSF) transgenic mice. Southern blot hybridisation analysis of Afl II-digested DNA purified from 4 lines of transgenic mice, as indicated. The left hand panel is an analysis of double stranded DNA digested with Afl II and probed with a 0.83 kb Afl II-Eco RI fragment of the GM-CSF gene. The right hand panel is an analysis of the same samples after denaturation at 99°C and rapid renaturation at 66°C before electrophoresis. The schematic below illustrates potential products and the probes employed to detect them. Sample preparation is described in the methods section.

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