Figure 1
From: Global turnover of histone post-translational modifications and variants in human cells
Experimental design. HeLa S3 cells were cultured in unlabeled 12C614N2-lysine media, and transitioned into labeled 13C615N2-lysine media. Daily samples were collected and analyzed by mass spectrometry (MS). MS spectra detected the 13C615N2-lysine labeled peptide (gray circle, H3 73-83) co-eluting with the respective unlabeled peptide (white circle) after the pulse at days 0, 1 and 6.