Dynamic instability of genomic methylation patterns in pluripotent stem cells

Table 1 Primer sets used in the experiments

Name	Sequence 5'→3'
Nested primers for bisulfite analysis of Mo-MLV^GFP long terminal repeat
GFPF1	TTTTTTTATATATTATTATTTATTTTTTTT
GFPR1	ATCAATCACTCAAAAAAAACCCTC
GFPF2	TAGGGTTAAGAATAGATGGAATAGTTGA
Bisulfite analysis of Oct4 promoter
Oct4F1	TGAGGAGTGGTTTTAGAAATAATTG
Oct4R1	AAACCAAATATCCAACCATAAAAAA
Oct4R2	CCAACCATAAAAAAAATAAACACC
Mo-MLV^42bp/GFP creation¹
Forward	TTATACTCCTCCACACACCATCACTCACTCTTTCTCAATCCA
Reverse	TGGATTGAGAAAGAGTGAGTGATGGTGTGTGGAGGAGTATAA

¹Moloney murine leukemia virus (Mo-MLV)^42bp/GFP was created by inserting this synthetic oligonucleotide into the U3 region of the Mo-MLV long terminal repeat.

ISSN: 1756-8935