Table 1 Oligos used in the assays
From: A combined HM-PCR/SNuPE method for high sensitive detection of rare DNA methylation
Gene name | Oligo name | Sequence 5'→3' |
|---|---|---|
SEPT9 | Forward | GATT-X-GTTGTTTATTAGTTATTATGT |
Reverse | AAATAATCCCATCCAACTA | |
Blocker | GTTATTATGTTGGATTTTGTGGTTAATGTGTAG-C3 | |
Probe | FAM-TTAACCGCGAAATCCGAC-BHQ1 | |
Oligo 27 | ttattagttattatgt | |
Oligo 28 | cggatttcgcggttaacg | |
Oligo 45 | cgcgttaaccgcgaaatcc | |
TMEFF2 | Forward | GGTTATTGTTTGGGTTAATAAATG |
Reverse | AAAAAAAAAAAACTCCTCTACATAC | |
Blocker | ACATACACCACAAATAAATTACCAAAAACATCAACAA-C3 | |
Probe | FAM-TTCGGACGTCGTTGTTCGG-BHQ1 | |
Oligo 30 | gttaataaatggagtt | |
Oligo 44 | gaacaacgacgtcc | |
Oligo 62 | cgtcgttgttcggt |