Figure 3

The Magoh2 promoter is a direct target for Mll2 and a model example of trxG /PcG opposition (part 2). ChIP analysis of the Magoh2 and Magoh promoters, as indicated at the top for the Magoh2 up, exon 1, down and Magoh exon 1 Q-PCR primer pairs as diagrammed in (Figure 2A). The first row of panels shows ChIP results obtained from TAP-tagged Mll2 ES cells using the TAP tag for immunoprecipitation compared with 5% of the input before immunoprecipitation. Foldness of Mll2-specific enrichment is noted at the side of each of the four panels. The second, third and fourth row of panels show fold change of chromatin immunoprecipitation using H3K4me3, H3K9me3 and H3K27me3-specific antibodies. Mll2F/F; Rosa26-CreERT2/+ (Mll2FC/FC)or Mll2F/+; Rosa26-CreERT2/+ (Mll2FC/+) cells were cultured for 12 days without (-) or with 4 days of 4-hydroxy tamoxifen for the last 4 days (d4), the middle 4 days (d8) or the first 4 days (d12).