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Table 1 Comparison of nucleosome positions on SNR6, mapped in this study with those reported in other studies

From: Multiple sequence-directed possibilities provide a pool of nucleosome position choices in different states of activity of a gene

No. Position (bps)
This study
Similar to
position (bp)
Comments Ref. No.
1. -545 to -368 -537 to -367 In vivo, in the presence of bound TFIIIC 39
2. -368 to -78 -367 to -214 In vivo, in the presence of bound TFIIIC 39
3. -656 to -453   In vitro, sequence-directed  
4. -453 to -241   In vitro, sequence-directed  
5. -241 to -78 -240 to -70 In vivo, in the presence of bound TFIIIC 37
6. -125 to +25 -194 to -16 In vivo, in the absence of bound TFIIIC 39
   -120 to +28 In vivo, under repression 37
7. -110 to +45 -116 to +40 Predicted 26
8. -90 to +65 Positions 8–10 together as bp -78 to + 110 in the plasmid
9. -60 to +95 p-539H6, Figure 1B, sequence-directed.
10. -40 to +115 Positions 9–15 together as bp -50 to + 316 in the plasmid
11. -20 to +125 pCS6, Figure 1C, sequence-directed.
12. +51 to +196 +48 to +192 In vitro, TFIIIC-dependent 3
   +94 to +198 In vivo, in the presence of bound TFIIIC 59
13. +71 to +216 +71 to +227 Predicted 26
   +85 to +206 In vivo, in the absence of bound TFIIIC 39
14. +91 to +236   In vitro, sequence-directed  
15. +101 to +256   In vitro, sequence-directed  
  1. All 15 positions in Column 2 are sequence-directed as nucleosomes are assembled in the absence of any transcription or chromatin assembly factors. Positions 1 to 5 are mapped by the low-resolution technique (Figure 1B) and fall in the upstream region; the rest of the positions on the gene region (6 to 15) are mapped by the higher resolution Exo III analysis (Figures 5 and 6) in this study. Positions 3 to 5, 8, 9, 14 and 15 represent the register R1 while R2 is constituted by the positions 1, 2 (or 3, 4), 6, 12 and 13. Arrangement of nucleosomes in active state R3 is generated by the positions 1, 2 (or 3, 4), 5 and 12 giving a nucleosome-free region flanked by positioned nucleosomes.