Nucleosome positioning properties of two SNR6 halves are different. (A) Schematic representation of the selected gene regions PCR-amplified from pCS6 and cloned into a plasmid along with their names and sizes is given. (B), (C) and (D) Indirect end-labeling analysis of the chromatin assembled over the plasmids 601c, 5' half and 3' half respectively. Arrows mark the positions of the genomic DNA ends while ovals mark the positioned nucleosomes. Lanes 1 and 2 show digestion pattern of naked DNA N, while digestion pattern of the chromatin samples C are shown in the lanes 3 and 4 in each panel. Probe was same as that in the Figure 1C while lane M shows molecular size markers.