Deletion of SGF73 results in a global increase in H2B ubiquitin levels similar to deletion of UBP8 , but does not affect H3 acetylation. A. Flag western blot analysis of H2Bub levels: Lane 1 H2BK123R, Lane 2 UBP8Δ, Lane 3 SGF73Δ, Lane 4 SGF73Δ, Lane 5 wildtype. The indicated bands represent FlagH2B, FlagH2Bub and FlagH2BubHA. B. Quantification of H3 lysine 9 Western blot signal demonstrates that deletion of SGF73 does not reduce global histone H3 lysine 9 acetylation when compared with deletion of GCN5.