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Figure 3 | Epigenetics & Chromatin

Figure 3

From: Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexes

Figure 3

Deletion of SGF73 results in the loss of the deubiquitination module from the SAGA/SLiK(SALSA) complexes. A. Silver stain of various SAGA/SLiK(SALSA) purifications. Lane 1 marker, Lane 2 Ada2Tap purification, Lane 3 Ada2TAP;sgf73Δ purification, Lane 4 Spt8Tap purification, Lane 5 Spt8TAP;sgf73Δ purification, Lane 6 SLiK(SALSA) purification from an HASpt7-1180TAP strain, where Spt7 lacks the C-terminus required for Spt8 association, Lane 7 HASpt7-1180TAP; sgf73Δ. The subunits that were used for purification are labeled, as well as the band corresponding to Sgf73. B. MudPit analysis reveals that deletion of SGF73 from Spt8, Gcn5 and HA-Spt7-1180TAP (Slik) purifications results in the loss of the deubiquitination module, ubp8, sgf11 and Sus1 from SAGA (numbers are total peptides obtained for each subunit).

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Epigenetics & Chromatin

ISSN: 1756-8935