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Figure 3 | Epigenetics & Chromatin

Figure 3

From: Repressive and non-repressive chromatin at native telomeres in Saccharomyces cerevisiae

Figure 3

Chromatin alterations towards the XIL centromere in a yku80 strain. (A) Frequency of fluoroorotic acid (FOA) resistance in Δyku80::KanMX strains containing the URA3-yEGFP marker adjacent to the core X element at the indicated telomere. The mean and standard deviation of FOA resistance is given for each strain. (B) The subtelomeric chromatin structure of the XIL telomere was analysed in the Δyku80::KanMX strain, by MNase digestion and indirect end labelling, using a probe adjacent to the BstX I site within yEGFP. The marker (M) was generated by digestion of purified DNA with BstX I and Pst I. An MNase hypersensitive site within the reporter open reading frame is indicated by an asterisk, other labelling is as for Figure 1B.

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