Biochemical interaction between the RNA Pol II carboxyl terminal domain and RNA silencing machinery components. (A) Whole cell extracts from 6-18 h wild type embryos were prepared. Native mouse serum was used in the control lane and 8WG16 monoclonal RNA Pol II antibody was used for pull down analysis. About 500 micrograms of lysate was used. AGO1 and Dicer-2 polyclonal antibodies (1: 1000) were used to perform western blot analysis. (B) Co-immunoprecipitation analysis performed similarly as above with H5 antibodies specific for RNA Pol II ser-2 phos.