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Figure 3 | Epigenetics & Chromatin

Figure 3

From: Epigenetic inheritance of an inducibly nucleosome-depleted promoter and its associated transcriptional state in the apparent absence of transcriptional activators

Figure 3

Mediator is not required for PHO5 transcription in the absence of activators in vivo. (a) Mediator is required for transcriptional initiation of PHO5 in vivo. Strains JKT0010 (WT), ROY010 (pho80), and ROY011 (pho80 srb4) growing in phosphate containing media (PHO5 repressed) were shifted to 39°C in low phosphate media, followed by analysis of PHO5 induction via the phosphatase activity assay. (b) Mediator is not required for continued transcription from the nucleosome-depleted promoter in the absence of activators. Strains JKT0010 (WT), JMY002 (spt6), and SKW067 (spt6 srb4) were shifted to 39°C while PHO5 transcription was occurring in low phosphate media, followed by the addition of phosphate as the signal to repress PHO5 transcription and analysis of PHO5 induction via the phosphatase activity assay.

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Epigenetics & Chromatin

ISSN: 1756-8935