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Figure 5 | Epigenetics & Chromatin

Figure 5

From: The fungus Neurospora crassa displays telomeric silencing mediated by multiple sirtuins and by methylation of histone H3 lysine 9

Figure 5

Histone H4 acetylation is increased in nst mutants. A) Western blots of nuclear extracts from strains N3120 (nst+) and N3132 (nst-1RIP 1nst-3RIP 1nst-5RIP 1) probed for the indicated epitopes. Different exposure times were used (not indicated) to avoid saturation of signal. B)In vitro NAD+-dependent deacetylase assays with the indicated H3 or H4 peptide substrates (NT, unmodified N-terminal peptide). Grey bars show activity for purified NST-1 protein; white bars represent GST (only) control.

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