Figure 4
Derepression of the subtelomeric transgenes by inhibitors of histone acetyltransferases and DNA methyltransferases. A) Nicotinamide (NAM) relieved silencing of telomeric hph (N2285) but not hph flanked by amRIP(N2015) at a non-telomeric site. All plates contained 0.6 mg/ml Hygromycin. Plates with NAM contained 0.1 mg/ml (middle plate) or 1 mg/ml (right plate) of the drug. Each plate received approximately 100,000 conidia. B) Approximately 1000 conidia from an nst+ strain (N3120) carrying the telVR hph allele were plated in the presence or absence of 0.5 mg/ml Hygromycin to assay the effect of the drugs 5-azacytidine (5AC), Trichostatin A (TSA) and NAM on telomeric silencing. C) Same as B except strain N3441 carrying the tel VIIL bar allele was plated in the presence and absence of 4 mg/ml Basta. Control plates lacking Basta show a lawn of growth.