Figure 3

Telomeric silencing depends on dim-5 , hpo and nst genes. Conidia from strains carrying the telomere VR hph allele were spotted at densities noted at the bottom of each panel to test sensitivity to moderate (A) or high (B) levels of Hygromycin. Strains used in A were: N2285 (nst⁺, "WT"); N2130 (nst-1^{RIP 1}); N2635 (nst-3^{RIP 1}). Strains used in B were: N3120 (nst⁺, "WT"); N2833 (dim-2); N2997 (dim-5); N3004 (hpo); N3125 (nst-1^{RIP 1}); N2667 (nst-2^{RIP 1}); N3126 (nst-3^{RIP 1}); N3130 (nst-5^{RIP 1}); N3132 (nst-1, nst-3, nst-5). C) Conidia from strains N3441 (nst⁺), N3447 (dim-2), N3449 (dim-5), N3443 (hpo), N3452 (nst-1^{RIP 1}), N3453 (nst-2^{RIP 1}), N3455 (nst-3^{RIP 1}), and N3445 (nst-5^{RIP 1}) were spotted on plates with or without Basta to assay expression of the bar transgene targeted to telomere VIIL. D) Conidia from strains N3457 (nst⁺) and N3456 (hpo) were spotted on plates with or without Basta to assay expression of the bar transgene targeted to telomere IIR.