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Table 1 Overview about all histone modifications and proteins examined and their nuclear localization in vegetative and differentiating cells.

From: Spatial and temporal plasticity of chromatin during programmed DNA-reorganization in Stylonychia macronuclear development

 

Vegetative cells

Differentiating cells

++ = high level

+ = moderate level

- = not detected

Micronuclei

Macronuclei

Micronuclei

Parental macronuclei

Macronuclear anlagen (a 1 to early a 2)

Macronuclear anlagen (a 2 to a 3)

Macronuclear anlagen (e)

Histone acetylation

       

H3K9ac

-

++

-

+ 4

++

++

++

H3K14ac

-

++

-

+ 4

++

++

++

H3K36ac

-

-

-

-

-

++

+ 4

Histone methylation

       

H3K4me1

-

++

-

+ 4

-

++

++

H3K4me3

-

++

-

+ 4

-

++

++

H3K9me3

-

-

-

+ 5

-

++

+ 4

H3K27me1

-

-

- (+ 1 )

-

-

++

+ 4

H3K27me3

++

-

++

-

-

++

+ 4

H3K36me3

+

-

+(- 2 )

-

-

+

+ 4

Histone phosphorylation

       

H3S28p

++

+

-

+ 4

++

++

++

Chromatin modifying proteins

       

E(z)/KMT6-like HMT

-

-

-

-

-

++

+ 4

Spdd1p

-

-

-

+ 5

-

++

+ 4

RNAi-related machinery

       

Piwi/mdp1

-

-

+ 3

++ 6

++

+ 4

+ 4

  1. 1transient accumulation during conjugation
  2. 2transient loss during conjugation
  3. 3transient accumulation early during conjugation
  4. 4signal intensity and frequency continuously decreasing
  5. 5modification occasionally observed as dispersed foci-like signals mostly not visible in stack projections
  6. 6resrtricted to conjugation and early exconjugant cells with no visible macronuclear anlagen