Figure 8

Chromatin-modifying proteins during macronuclear differentiation. In situ antibody staining of a putative E(z)/KMT6-like protein (A, B) and the HP1-homolog Spdd1p (C, D) (green). To-Pro-3 was used for DNA counterstaining (red). The cellular shape was visualized using an α-tubulin-antibody (grey). Confocal image stack projections. (A) In macronuclear anlagen the E(z)/KMT6-like protein is not detected at stage a_1–2, but accumulates subsequently (a₃) and is present at stage e during DNA elimination. (B) The E(z)/KMT6-like protein co-localizes with chromosomal bands in spread polytene chromosome preparations. (C) Spdd1p is not detected in early macronuclear anlagen (a_1–2). (D) Spdd1p accumulates in advanced stages (a₃) during macronuclear development.