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Figure 6 | Epigenetics & Chromatin

Figure 6

From: Spatial and temporal plasticity of chromatin during programmed DNA-reorganization in Stylonychia macronuclear development

Figure 6

Histone modifications in macronuclear anlagen during DNA elimination. Nuclear types occurring during DNA elimination: In macronuclear anlagen (e) at this stage bulk micronuclear sequences are removed resulting in a DNA-poor nucleus containing less than 10% of micronuclear sequences. Further micronuclei (m) and fragmented parental macronuclei (p) are found in the cells. In situ antibody staining (color code as described in Figure 2): Histone modifications detected at stage a3 of advanced macronuclear anlagen are also detected at stage e during DNA elimination. Importantly, signal intensity of H3K36ac (F) reaches a maximum at the transition from a3-e, and signal intensity of H3K36me3 (E), H3K9me3 (F), H3K27me1 (G), and H3K27me3 (H) gradually decreases. Subsequently, all these modifications (E-I) can no longer be detected in prospective macronuclei.

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