Figure 7
RNA fluorescent in situ hybridisation analysis of Xist / Tsix expression in DicerΔ/Δ embryos at E6.5. (A) RNA FISH analysis of Xist expression in representative whole mount E6.5 wt and Dicer-deficient embryos using a full-length DIG labelled Xist probe. The probe is detected with a FITC-coupled antibody (green). Examples show combined confocal optical sections through the whole embryo (15 sections with a distance 0.35 μm between each section were merged for each embryo; 63× objective). (B) Enlarged view (×3) of the epiblast part of the whole mount E6.5 wt and Dicer-deficient embryos after RNA FISH with Xist probe shown in (A). The Xist probe is detected with a FITC-coupled antibody (green) and DNA is counterstained with DAPI. (C) RNA FISH analysis of Xist (green) and Tsix (red) expression in whole mount E6.5 wt and Dicer-deficient embryos. Examples show combined confocal optical sections through the epiblast part of the embryo (10 sections with a distance 0.35 μm between each section were merged for each embryo). Pinpoint Xist/Tsix signal (arrow) is visible in wt and in DicerΔ/Δ embryos.