Recognition imaging of native CenH3 core particle complexes. CenH3 core particles obtained by hydroxylapatite enrichment of chromatin followed by immunoprecipitation were visualized by an anti-CenH3 atomic force microscopy tip (A). The recognition signal is detected as dark spots in the recognition image (B). In the overlay shown in (C), the recognition signal is marked with green dots for visual clarity using custom software. No recognition signal is seen after incubation with a peptide corresponding to the CenH3 epitope (D). The bar is 100 nm and the Z range and volt scale are shown at the bottom of the images.