Fig. 3
From: The N-terminal dimerization domains of human and Drosophila CTCF have similar functionality
Results of the Y2H assay of the dimerization activity of the proximal and distal parts of DD from dCTCF. (A) Multiple sequence alignment of the N-terminal DD regions from different Drosophila species. Color intensities indicate percentage identity. Secondary structures were predicted with AlphaFold (https://alphafold.ebi.ac.uk/): green arrows represent putative beta sheets, and the red line represents the alpha helix. (B) AD represents the GAL4 activation domain, and BD stands for the GAL4 DNA binding domain. The ‘+’ interaction indicates the ability of yeast to grow on assay plates without histidine