Fig. 1
From: The N-terminal dimerization domains of human and Drosophila CTCF have similar functionality
Mutations in the CTCF gene. (A) Schematic representation of dCTCF replacement. The untranslated regions of the CTCF gene are shown as grey arrows, and the coding region is marked with a blue line. The endpoint of the CTCFattP(mCh)platform is shown with breaks in the black line. The attP, loxP, and attB sites are shown as white, orange, and green arrows, respectively. Other designations are shown in the figure. The schematic representation of full-length restored dCTCF-HA (wt-HA) is shown below. The deletion variants of the CTCF gene are shown as black lines with breaks corresponding to the deleted domains. (B) Immunoblot analysis (6% SDS PAGE) of protein extracts prepared from adult flies of dCTCFwt-HA (wt-HA), dCTCFΔ2–80-HA (Δ2-80-HA), dCTCFΔ132–170-HA (Δ132–170-HA), dCTCFΔ80–170-HA (Δ80–170-HA), dCTCFΔ80–125-HA (Δ80–125-HA), dCTCFΔ171–243-HA (Δ171–243-HA), and dCTCFΔ171–287-HA (Δ171–287-HA) lines with anti-HA and anti-lamin Dm0 (internal control) antibodies