Fig. 1From: Analysis of long-range chromatin contacts, compartments and looping between mouse embryonic stem cells, lens epithelium and lens fibersTissues and experimental design of the Hi-C study. Lenses were harvested from newborn (P0.5) mice and microdissected into epithelium and fiber cells (30 lenses x 2 replicates). Mouse ES cells were harvested near ~ 80% confluency (2.0 × 106 cells x 2 replicates). Cells were crosslinked and processed according to Arima’s library preparation protocol. Chromatin contact maps were generated using the ENCODE pipeline. Differential compartment A/B analysis was performed using dcHiC (see Materials and Methods). The principal component analysis (PCA) on compartmental eigenvectors shows that both lens cells are distinct from the ES cellsBack to article page