Fig. 7

The disruption of PCGF6/MAZ/CDK4 axis inhibits pRCC xenograft progression in vivo. A Tumor volumes were measured in nude mice with xenograft of Caki-2 cells with stable deletion of MAZ or overexpression of PCGF6, alone or together. B The representative tumor size of all mice is presented. C Measuring wet weight of xenograft tumors. D The DNA methylation condition of the CpG island of the MAZ promoter in xenograft tumors were examined using BSP. E The protein levels of MAZ, PCGF6, and CDK4 were measured in tumors using Western blotting. F Quantitative analysis of Western blotting data from E. G Double immunofluorescence staining measures the level of MAZ and CDK4 in xenograft tumors. H Quantitative analysis of fluorescence intensity of G. All data are from three independent experiments and are expressed as mean ± standard error. *p < 0.05, **p < 0.01, ***p < 0.001 versus the corresponding controls