Fig. 5

The SMC5/6 accumulation correlates with the H3K9 acetylation status. A Heatmap statistical analysis of the loci with reduced SMC5/6 occupancy upon the gcn5 deletion. The Nse4-FLAG signals from WT and Δgcn5 are compared, and their differential plot is shown in the middle panel (Δgcn5-WT). The results from ChIP-seq analysis of H3 and H3K9ac are shown (H3K9ac/H3). In addition, transcriptomic (RNA-seq) data are included (mRNA). The genes (normalized to 1 kb width) and their surrounding regions (500 bp upstream of TSS and 1 kb downstream of TTS) are shown. B Scatter plot analysis shows a strong correlation between the drop of SMC5/6 accumulation upon gcn5 deletion (X-axis) and H3K9-acetylation status (Y axis) of gene regions (and their transcription levels; colour scale). C The results of chromatin immunoprecipitation followed by quantitative PCR (ChIP–qPCR) at selected gene loci are shown. Strains 503 (neg. control), MMP21 (WT, containing Nse4-FLAG), Nse4-FLAG Δgcn5 (Δgcn5) and Nse4-FLAG gcn5-E191Q (gcn5-E191Q) were analysed. The fold enrichment was calculated against the negative slx9 locus (mean ± standard deviation, n ≥ 3 biological replicates). The unpaired Wilcoxon statistical test was used: ns, non-significant; *p < 0.05