Fig. 1

PDS5A and PDS5B localize to shared sites across the mESC genome. A Genome browser tracks of PDS5A, PDS5B, RAD21, and CTCF ChIP-seq signal near the promoter of Sox2 in WT mESCs (Z-score normalized). B Average signal plots of PDS5A and PDS5B ChIP-seq signal at all PDS5A peaks and all PDS5B peaks (Z-score normalized). C Number of PDS5A and PDS5B peaks overlapping relevant functional elements across the genome: CTCF sites, enhancers, promoters, or other (none of the above). D Clustered heatmaps of PDS5A, PDS5B, RAD21, and CTCF ChIP-seq signal at a union list of PDS5A and PDS5B peaks, ordered by RAD21 signal (k-means = 3) (Z-score normalized). E Western blot analysis following co-immunoprecipitation of IgG (negative control), PDS5A, PDS5B, and RAD21 in WT nuclear lysates, under low and high stringency conditions