Fig. 3

Influence of PARylatyion on TET1 activity in vitro. a Kinetics of TET activity of: in vitro PARylated TET1 (upper graph) and TET1 in control reactions (bottom graph). The level of 5hmC produced by TET1 was plotted as a function of time and exponentially fitted using the least square fit. The data points are shown as mean ± SEM (n = 2). b TET1 activity represented by initial reaction velocity of 5mC oxidation by: in vitro PARylated TET1 (upper graph) and TET1 in control reactions (bottom graph). Initial reaction velocities were calculated by linear regression based on the signal at the initial time points of TET activity kinetics. Results were scaled to TET1 control and shown as mean ± SEM (n = 2). TET1 control—unmodified TET1; TET1(-PARP-1) ± NAD⁺—TET1 mock PARylated with dialysis buffer instead of PARP-1 with or without NAD⁺; TET1 + PARP-1(-NAD⁺)—TET1 mock PARylated with PARP-1 without NAD⁺; TET1 + PARP-1 + NAD⁺—PARylated TET1. n‐number of independent experiments, a.u.-arbitrary units