Fig. 5

Gene body methylation and gene expression variability in P. fallax. a Comparative analysis of gene body methylation patterns in P. virginalis and P. fallax. b Species-specific differential gene body methylation. The heatmap shows differentially methylated genes (DMGs) with an average species-specific methylation difference > 0.1. c P. fallax has a significantly higher average gene body methylation level than P. virginalis (P < 2.2e⁻¹⁶, two-tailed t test). d Comparison of gene expression variation between marbled crayfish and P. fallax. Coefficients of expression variation are indicated for triplicate RNA-seq datasets from the abdominal musculature. The difference between the two species is highly significant (P < 5.58e⁻¹³, two-tailed t test)