Fig. 6
From: Internal modifications in the CENP-A nucleosome modulate centromeric dynamics
K124A/Q mutant cells have higher rates of mitotic defects compared to wild-type CENP-A. Cells were transfected with GFP-tagged CpA, K124A or K124Q, and synchronized with a double thymidine block to enrich for mitotic cells. Synchronized cells were immuno-stained for αTubulin, and images captured, binned and quantified into four categories: normal mitosis, mitosis with lagging chromosomes (white arrow), multipolar spindles, and multipolar spindles + lagging chromosomes (white arrow). Percentage of cells in each category for each mutant is shown graphically and values stated in the merge panel