Fig. 3
From: Imprinted DNA methylation reconstituted at a non-imprinted locus
The Wnt1DR allele recapitulates partial DNA germline and complete somatic imprinted DNA methylation patterns. a Methylation levels at Wnt1DR in two DR/DR sperm samples and two pools of fifty DR oocytes as assayed by bisulfite sequencing. b Distribution of methylated CpGs within a paternal lineage F6 animal from (c), a maternal lineage F6 animal from (c), and the two sperm samples from (a). c Left mouse pedigrees with maternal (M), paternal (P), or alternating (A) transmission of Wnt1DR. Right DNA methylation levels, as assayed by bisulfite sequencing, using one to four animals sampled from the pedigrees. Circles, females; squares, males; diamonds, either sex; half-filled shapes, heterozygous animals; NM not measured. d High-throughput bisulfite analysis of extra embryonic (EE) tissue, head, spine, and internal organs of e9.5 embryos from either maternal (DR/+) or paternal (+/DR) transmission