Fig. 3

Chromatin profile and transcriptional activity of β-globin domain. a Schematic of the β-globin domain, detailing the developmentally regulated β-globin genes and DNAse I-hypersensitive sites (HS4 and 3′HS delimitating the locus). HS1, HS2, HS3 and β^A/ε enhancer are collectively known as locus control region (LCR) and regulate the expression of the four β-globin genes. Below the maps, are signal tracks showing DNA enrichment in F1 fraction, CTCF-binding sites (as vertical bars), transcripts on (+) and (−) strands and H3 modifications. mRNAs (with exons as black boxes) are shown below their template strand. The inset to the right shows the level of transcripts on an expanded scale. Vertical blue lines illustrate the position within the domain of prominent features (H3K27ac and/or H3K4me3 peaks and eRNAs). b Amplification of signal tracks showing F1-enriched DNA and transcribed RNAs in the β-globin LCR region