Fig. 5From: Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defectsDistinct alterations in homeobox gene transcription arising during and after the window of EtOH exposure. Neural stem cells were cultured in the presence of 160 or 240 mg/dL EtOH for 3 days, followed by a 4-day recovery in media lacking EtOH. Cells were harvested at days-3 and 7, and transcript levels measured using RT-qPCR. Graphs represent three independent replicates (N = 3), with two independent RT reactions and three qPCR measurements for each RT. Differences were measured using a one-way ANOVA, error bars represent SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001Back to article page