Fig. 3

In vitro EtOH exposure does not inhibit methyltransferase enzymatic activity, but does induce alterations in the transcriptional control of DNA and Histone methyltransferase enzymes. a, b Measures of histone methyltransferase activity in EtOH-exposed neuroepithelial stem cells. Cells were cultured in the presence of 80–240 mg/dL EtOH for 3 days, and cellular extracts assayed for a H3K27 and b H3K9 histone methyltransferase activity using a colorimetric assay. Differences were measured using a one-way ANOVA. Error bars represent SEM. N = 3. c Measurement of transcripts encoding enzymes governing DNA, H3K9, and H3K27 methylation. Primary neuroepithelial stem cells were cultured in the presence of 160 or 240 mg/dL EtOH for 3 days, followed by a 4-day recovery in media lacking EtOH. Samples were harvested at days-3 and 7, and transcript levels determined by RT-qPCR. Graphs represent three independent biological replicates (N = 3), with two independent RT reactions and three qPCR measurements for each RT. Significance was measured using a one-way ANOVA, error bars represent SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001