HP1β-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs
© Stixová et al.; licensee BioMed Central. 2014
Received: 11 April 2014
Accepted: 12 December 2014
Published: 30 December 2014
The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways.
We analyzed the DNA-damage response after ultraviolet A (UVA) and γ irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1β (HP1β) level. Moreover, Förster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1β that was dependent on a functional chromo shadow domain of HP1β. Thus, overexpression of HP1β with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin. Transcription factor UBF1 also interacted directly with DNA inside the nucleolus but no interaction of UBF1 and DNA was confirmed outside the nucleolus, where UBF1 recruitment to DNA lesions appeared simultaneously with cyclobutane pyrimidine dimers; this occurrence was cell-cycle-independent.
We propose that the simultaneous presence and interaction of UBF1 and HP1β at DNA lesions is activated by the presence of cyclobutane pyrimidine dimers and mediated by the chromo shadow domain of HP1β. This might have functional significance for nucleotide excision repair.
KeywordsDNA-damage response DNA repair Irradiation Live-cell studies Nucleolus UBF1
Genome injury by radiation or pollutants affects cellular metabolism, cell cycle, proliferation, and apoptosis, and activates DNA repair pathways. Genotoxic agents can injure DNA and induce changes in chromatin conformation. Activation of DNA repair events is associated with rearrangement of nuclear compartments, including nucleoli, nuclear bodies, and foci of accumulated proteins[1–3]. Cell-cycle control and the DNA-damage response (DDR) are also regulated by nucleolar proteins, many of which are responsible for maintaining nuclear architecture and cellular shape. For example, ultraviolet irradiation induces rearrangement of nucleolar proteins Ki-67 and WRN as well as relocation of inhibitor of growth protein 1 from the nucleoplasm to the nucleolus (; summarized by[1, 5]). Rubbi and Milner suggest that the nucleolus is a stress sensor that guarantees the optimal level and nuclear distribution of p53 and that this functionality can be disrupted by genome injury. Kurki et al. showed that ultraviolet damage induces relocation of nucleophosmin from the nucleolus to the nucleoplasm, where it interacts with p53 and HDM2 and thereby stabilizes the level of p53. These results indicate that the nucleolus is an important organelle that is sensitive to genome injury and serves unique DNA-damage-related functions. This characterization is based on the observed nucleolar protein mobility and unique repair processes of ribosomal genes[3, 8]. Moreover, Kruhlak et al. showed that ionizing radiation inhibits RNA polymerase I (RNA pol I) activity, which substantially affects ribosomal gene transcription.
The nucleolus is a highly compartmentalized nuclear region consisting of a fibrillar center, a dense fibrillar component, and a granular component[10–12], which can separate after genome injury. Nucleolar proteins, including upstream binding factor 1 (UBF1), function specifically in ribosomal biogenesis[1, 10, 14, 15]. Moreover, Moore et al. showed that the nucleolar proteome, especially UBF1 foci, becomes highly reorganized after cell exposure to UVA irradiation. The nucleolar DDR is strikingly different between ultraviolet-irradiated and γ-irradiated genomes, consistent with different DNA lesions that occur in response to ultraviolet and ionizing radiation. For example, cyclobutane pyrimidine dimers (CPDs) or 6-4 photoproducts, among others, are preferentially induced by UVA radiation and recognized by the nucleotide excision repair pathway, whereas double-stand breaks (DSBs) mostly appear as secondary lesions after γ-irradiation. The DSB-containing DNA lesions are recognized by proteins involved in non-homologous end-joining or homologous recombination repair pathways. These processes can be also initiated in ribosomal DNA because the nucleolar proteome consists of proteins involved in DSB-related repair pathways, such as ataxia telangiectasia mutated (ATM), ataxia telangiectasia and Rad3-related protein (ATR), MRE11, PARP1, and KU70/80.
In this study, we investigated whether proteins involved in the DDR occupy the nucleolar UBF1-positive compartment, focusing particularly on 53BP1 and γH2AX. We also examined whether UBF1 and other selected nucleolar proteins, including fibrillarin, TCOF, RPA194, and HP1β[17, 18], co-localize with locally induced DNA lesions, which are characterized outside the nucleolus by heterochromatin protein 1β (HP1β) accumulation[19, 20]. We analyzed the distribution pattern of UBF1 at DNA lesions in live and fixed cells. Moreover, we assessed whether UBF1 recruitment to DNA lesions is cell-cycle-dependent and which DNA repair pathway is engaged in this process. Next, we examined how inhibition of ribosomal gene transcription influences UBF1 status after DNA damage, and we used Förster resonance energy transfer (FRET) to assess whether UBF1 protein binds directly to DNA or interacts with other proteins recruited to UVA-damaged chromatin. Our results show a pronounced accumulation of UBF1 at locally induced DNA lesions. Outside the nucleolus, UBF1 appeared in parallel with CPDs and interacted with accumulated HP1β at DNA lesions but did not interact with DNA. However, inside the nucleolus, UBF1 as a transcription factor binds directly to DNA. Interaction was also observed between UBF1 and HP1β, the level of which was not increased in the nucleolus after UVA irradiation. These observations suggest an existence of different DNA repair mechanisms in ribosomal genes and chromatin outside the nucleolus.
Localization of DDR-related proteins and UBF1 in UVA-irradiated genomic regions
However, we recently showed that HP1β not only occupies chromatin outside the nucleolus, but subtle amount of HP1β can be also detected in ribosomal genes by chromatin immunoprecipitation PCR. Using the same experimental approach, we also observed a high level of γH2AX at ribosomal genes of non-irradiated cells. Therefore, in this study, we not only analyzed UBF1 as an important nucleolar protein but also studied additional nucleolar proteins, including HP1β and γH2AX, and their potential to be recruited to nucleolar DNA lesions. We also studied the presence of RNA pol I subunit RPA194, TCOF, and fibrillarin at DNA lesions (Additional file1: Figure S1) by subjecting cell nuclei to local microirradiation. DNA lesions were identified by markedly increased GFP-HP1β fluorescence, but RPA194, TCOF, and fibrillarin were not recruited to UVA-damaged chromatin (compare irradiated cells in Additional file1: Figure S1 with non-irradiated cells in Additional file2: Figure S2Aa-d).
UBF1 status at DNA lesions with respect to cell cycle phases and DNA repair pathways
Next, we analyzed UBF1 involvement in DNA repair pathways. We used a UVA laser (355-nm wavelength) to induce DSBs, which are recognized by non-homologous end-joining or homologous recombination repair mechanisms. To confirm the induction of DSBs, we analyzed γH2AX- and 53PB1-positivity in irradiated regions (Figure 1D,E,[20, 22]). Additionally, we tested possible activation of the nucleotide excision repair pathway by a UVA laser (355-nm wavelength, either with or without 5-bromo-2′-deoxy-uridine (BrdU) sensitization; see Methods section) and observed an increased appearance of CPDs (Figure 2B,Ca). We also used a 405-nm UVA laser to specifically induce γH2AX- or 53BP1-positive DSBs but not CPDs (, Figure 2Cb,Cc). The results of this experiment show that UBF1 was only recruited to chromatin with CPDs, suggesting that UBF1 is involved in the nucleotide excision repair pathway.
UBF1 status at DNA lesions after suppression of transcription
UBF1 interaction with selected DDR-related proteins
Overexpression of HP1βΔCSD, but not HP1βΔhinge or HP1βΔCD, has a dominant-negative effect on UBF1 recruitment to DNA lesions
UBF1 binds to DNA in only nucleolus
FRET analysis showed that UBF1 binds to TO-PRO-3-stained DNA in the nucleolus but not to non-ribosomal DNA (Figure 7Ba,Bb). These experiments demonstrate that UBF1 functions as a transcription factor in ribosomal genes because of its binding to DNA in the nucleolus. This was observed in the non-irradiated and UVA-irradiated genome (see quantification in Figure 7Bb). FRET analysis not only showed that UBF1 interacted with HP1β (Figure 4E) but also confirmed UBF1 binding to ribosomal DNA (Figure 7Ba,Bb). These results also serve as verification of our FRET analyses.
Next, we examined the interaction of UBF1 and HP1β in entire nucleus using immunoprecipitation (Figure 7C). By this experimental technique, we confirmed the interaction between HP1β and UBF1 (reference interacting partners were HP1β and H3K9me3). Moreover, in the presence of ethidium bromide (EtBr), which protects DNA from binding to protein complexes, we did not observe an interaction between HP1β and UBF1. This event should be related to ribosomal genes, which are occupied by UBF1. Therefore, UBF1 protein-DNA and UBF1-HP1β interactions might play a role not only in the transcription of ribosomal genes but also in DDR-related events in the nucleolus (; Figures 4E, and7B,C).
Together, our data show that UBF1 is recruited to DNA lesions, owing to its interaction with HP1β. This DDR-related event is likely dependent on a functional CSD of HP1β. Moreover, as expected in the nucleolus, the transcription factor UBF1 binds directly to DNA. From a functional point of view, it is possible that radiation induces UBF1 release from promoter regions of transcribed genes. Therefore, UBF1 may accumulate at sites of DNA damage because of an affinity for altered chromatin topologies.
Our findings indicate that UBF1 recruitment to DNA lesions is a unique DDR of this transcription factor and not an artifact of GFP tagging or transient transfection. This conclusion is based on results of immunofluorescence staining of endogenous UBF1, which co-localized with DNA lesions (Figure 1B). Moreover, Western blot analysis detected a weaker signal for recombinant UBF1 than for endogenous UBF1 (Additional file2: Figure S2B). This shows that our transient cell transfection for UBF1 visualization by GFP was successfully optimized and did not induce apoptosis, as we detected no lamin B fragmentation (Additional file2: Figure S2B).
In this study, we investigated genome responses to ultraviolet and γ-radiation and accumulation patterns of UBF1 and HP1β at UVA-damaged chromatin in live cells. Radiation is considered a genotoxic agent that induces genomic DNA lesions accompanied by changes in nuclear and nucleolar morphologies. Different types of radiation can cause nucleolar segregation, nuclear relocation of proteins, and changes in protein post-translational modifications[4, 6, 8, 23–28]. Ionizing radiation can induce nucleolar disruption, leading to ATM-dependent inhibition of RNA pol I activity, thereby suppressing ribosomal gene transcription[6, 9]. A similar silencing effect is ascribed to actinomycin D, which primarily inhibits RNA pol I activity and induces dose-dependent single-strand breaks[29, 30]. Here, actinomycin D delayed UBF1 recruitment to nucleoplasmic DNA lesions (Figure 3B).
Our data are consistent with those of Moore et al., who observed radiation-induced rearrangement of UBF1-positive nucleolar regions, and demonstrate that some nucleolar proteins, including UBF1, respond to DNA injury and relocate in a damage-specific manner (Figures 1A and2B,C). Here, we show for the first time the direct recruitment of endogenous and recombinant UBF1 to UVA-damaged chromatin (Figure 1A,B). We determined that UBF1 but no other nucleolar proteins, such as RPA194, TCOF, or fibrillarin co-localized with locally induced DNA lesions (Figure 1A,B, and Additional file1: Figure S1A-C). UBF1 accumulated at DNA lesions in G1 and G2 cell-cycle phases, and interacted with other DNA-damage-related proteins, such as HP1β (Figures 1B,2A, and4E). Moreover, overexpression of HP1βΔCSD had a dominant-negative effect on UBF1 recruitment to DNA lesions (Figure 6A-C).
Our results are also consistent with those of Luijsterburg et al., who showed the importance of CSD for HP1β recruitment to DNA lesions. Here, we additionally specified HP1β functioning at DNA lesions by its interaction with UBF1 (Figures 1B,4E, and6A-C). Moreover, we showed that recruitment and interaction of HP1β with UBF1 at DNA lesions is a specific event appearing simultaneously with CPDs (Figure 2C), recognized by the nucleotide excision repair pathway[31, 32]. This process is not accompanied by UBF1 dimerization at DNA lesions (Figure 4B). Moreover, UBF1, as a transcription factor for ribosomal genes, interacts with DNA only in the nucleolus but not outside nucleolar regions in non-irradiated and UVA-irradiated cells (Figure 7Ba,Bb).
Emerging evidence indicates that HP1 isoforms have a role in the DDR and here we showed the same for UBF1. It is well known that HP1 isoforms can be recruited to ultraviolet-induced DNA lesions, oxidative lesions, and ionizing radiation-induced DNA breaks. Luijsterburg et al. showed that HP1 homologues are recruited to UVA-damaged chromatin, positive on CPDs, simultaneously with γH2AX- and 53BP1-positive DSBs. Similar results were found in the present study for CPDs, 53BP1, and γH2AX when we used irradiation by 355-nm UVA laser (Figures 1D,E and2B). However, 405-nm UVA laser irradiation did not induce CPDs, but only 53BP1 (Figure 2Cc) or γH2AX positivity. This experimental approach revealed an association of UBF1-HP1β with only CPDs, but not with DSBs. Interestingly, Zarebski et al. documented the appearance of HP1 isoforms at sites of oxidative damage together with OGG1 and XRCC1, which are factors involved in base excision repair. According to these findings, it seems evident that the recruitment of HP1β, and possibly UBF1, should be assessed in a manner sensitive to the complexity of several simultaneous DNA repair pathways, as suggested by Dinant and Luijsterburg.
As UBF1 is a ribosomal gene transcription factor, the theory of transcription-coupled repair (TCR) (summarized by[31, 35]) is relevant to our investigations. We showed that UBF1 colocalizes with CPDs, which are recognized by the nucleotide excision repair pathway, and it is well known that TCR is a variant of nucleotide excision repair. The TCR pathway is associated with protein-coding genes, but we observed pronounced UBF1 recruitment in the nucleolus, where ribosomal genes reside (see detail of UBF1 and nucleoli in Figures 1A,B and3C). The TCR model assumes that RNA pol II is arrested when the transcriptional machinery recognizes DNA lesions. This stalling of the massive transcription apparatus effectively signals to DNA repair proteins through the Cockayne syndrome B or A proteins, which also function during ribosomal gene transcription. Thus, DDR and ribosomal gene transcription converge at this point. In the present study, we also must consider the possibility that DSBs might be recognized by signaling pathways other than DNA repair pathways. For example, Ju et al. and Haffner et al. report that strand breaks may be a part of other genomic processes, including transcription. In some cancer cells, the transcriptional program of fundamental genes involves the formation of DSBs, and recruitment of DSB-related repair proteins can appear in parallel with the relocation of chromatin loops of activated genes to transcription sites, called transcription potentially factories. This transcription-related event requires topoisomerase II β activity, which is responsible for DNA coiling (summarized by). Moreover, our results allow the possibility that other transcription factors can be also recruited to DNA lesions (Figure 1A,B;).
The results of our study indicate that the nucleolus and the nucleolar UBF1 protein are important components of the cellular response to genome injury[1, 41]. The phenomenon of DNA-damage-induced nucleolar segregation is well documented[23, 42]. Thus, the responses of nucleolar proteins to radiation and radiation-induced changes in nucleolar morphology should be an area of study with respect to tumor radiotherapy. Our data show that the nucleolar UBF1 protein interacts with HP1β, and that both proteins co-localize with CPDs. This radiation-induced event can be influenced by overexpression of HP1βΔCSD, which has a dominant-negative effect on UBF1 recruitment to DNA lesions, and this DDR could be associated with extensive chromatin conformational changes induced by UVA irradiation. Together, our results indicate that UBF1 recruitment to DNA lesions probably depends on CPDs and the presence of HP1β at UVA-damaged chromatin, as shown by immunofluorescence, FRET analysis, and siRNA experiments (Figures 2C,4E, and5B,C).
Cell cultivation and transfection
The iMEFs were a generous gift from the laboratory of Professor Thomas Jenuwein (Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany). These cells were used as wild-type controls and were cultivated in DMEM with 10% FBS and appropriate antibiotics at 37°C in a humidified atmosphere containing 5% CO2. The iMEFs were immortalized according to the following standard protocol: every third day, the cell culture was split and counted, and 3 × 105 cells were transferred to a new 10-cm plate. Immortalization occurred through natural selection; cell irradiation was not used. The results obtained from iMEFs were verified in primary MEFs isolated from 12.5-day embryos of ICR mice according to the method of Bártová et al.. ICR mice were obtained from the Breeding Facility of the Medical Faculty, Masaryk University, Brno, Czech Republic. The MEF isolation was performed according to an experimental protocol confirmed by the National and Institutional Ethics Committee (protocol No. 224/2012). Mice were kept under standard conditions, and their use followed the European Community Guidelines of accepted principles for the use of experimental animals. Mice were sacrificed by overexposure to anesthetics (Narcamon/Rometar solution, Spofa, Czech Republic). For additional analysis, we used 3T3 cells stably expressing GFP-HP1β (a generous gift from Dr. Paul Verbruggen, Swammerdam Institute for Life Sciences, University of Amsterdam, Netherlands). Dr. Martijn S. Luijsterburg (Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden) generously provided plasmids encoding GFP-HP1β-ΔCSD, GFP-HP1β-Δhinge, and GFP-HP1β-ΔCD. For studies on cell-cycle-dependent recruitment of UBF1 proteins to DNA lesions, we used HeLa-Fucci cells expressing RFP- Cdt1 (red fluorescence) in the G1 phase and GFP-Geminin (green fluorescence) in the G2 phase.
For selected experiments, cells were treated with 0.5 μg/ml actinomycin D (#A9415, Sigma-Aldrich) for 2 h. For transfection of iMEFs, cells were cultivated on glass-bottomed tissue culture dishes to 70% confluence, and transfected with 2 to 5 μg plasmid DNA encoding GFP-UBF1 (#17656; Addgene, Cambridge MA, USA), GFP-p53 (#12091; Addgene, Cambridge MA, USA). Transfections were performed using METAFECTENE™PRO reagent (#T040-2.0, Biontex Laboratories GmbH, Germany). These methodological parameters were used for all cell transfection experiments.
Induction of DNA lesions and confocal microscopy
For local microirradiation using a UVA laser (355-nm or 405-nm wavelength), cells were seeded on uncoated, γ-irradiated, 50-mm glass-bottomed dishes used for inverted microscopy (No. 0; MatTek Corporation, USA, #P50G-0-30-F) or 35-mm grid-500 μ-dishes (#81166, Ibidi, Germany). At 70% confluence, the cells were sensitized with BrdU according to the method of Šustáčková et al. and subsequently UVA irradiated. BrdU incorporation was performed to increase the radiosensitivity of cells. These experiments were performed using a previously described protocol. Briefly, cells were sensitized with 10 μM BrdU for 16 to 18 h before local microirradiation using a 355-nm UVA laser (all figures except Figure 2Ca-c). To control BrdU incorporation, cells were stained using the BrdU labeling and detection kit I (#11296736001, Roche, Prague, CZ) (not shown). We also examined DDR events without BrdU sensitization[40, 47] and observed low-level GFP-UBF1 recruited to sites of DNA damage (Figure 2Ca) similar to that described by Bártová et al. for GFP-OCT4 recruitment to DNA lesions. Irradiation using a 405-nm UVA laser was performed here without pre-sensitization (Figure 2Cb,Cc). Usually, cell treatment by Hoechst 33342 (Invitrogen) for 5 min is applied before irradiation by a 405-nm UVA laser[19, 20], but we did not apply this experimental step, in order to be closer to the induction of a single DNA repair pathway. Another experimental approach is to apply different intensities of a 405-nm laser for cell irradiation. This was not used here because our laser setting was optimal for our analyses.
In all cases, in the present experiments, defined regions of interest (ROIs) in the nucleolus and outside the nucleolar region were irradiated by 100% laser power for 4 s. Laser intensity was not reduced at the acousto-optic tunable filter. No protein accumulation at UVA-damaged chromatin was considered when fluorescence intensity was identical at irradiated and non-irradiated regions. We considered protein accumulation as a fluorescence intensity higher than 30% of standard cellular level.
The UVA irradiation was performed using a Leica SP5 X confocal microscope at the following settings: 512 × 512 pixels, 400 Hz, bidirectional mode, 64 lines, zoom 8 to 12. We used the following settings for confocal scanning: 1024 × 1024 pixels, 400 Hz, bidirectional mode, 4 lines, zoom 8 to 12. Before immunofluorescence staining, live cells were monitored in real time, and ROIs were selected and irradiated. When increased levels of proteins of interest appeared, cells were fixed in 4% formaldehyde for 10 min, and immunostaining was performed. We irradiated approximately 10 nuclei per microscope dish (for each experiment, we used at least three dishes), and the whole procedure took approximately 30 min. After immunodetection, locally irradiated cells were monitored according to defined coordinates marked on the microscope dishes.
In experiments involving γ-irradiation of the whole cell population, cells were irradiated with 5 Gy of γ-rays using a 60Co source, and analyses were performed 2 h after γ-irradiation. No pre-sensitization was used.
Specification of radiation sources
UVA laser, 355 nm
Laser from Coherent, Inc.; laser power, 80 mW; irradiated area, 25.8 × 10-8 cm2; irradiation time, 4 s; resolution for image acquisition, 512 × 512; line average, 64; pixel size, 60.06 × 60.06 nm; image size, 30.74 × 30.74 μm; total number of irradiated pixels, 24,455; irradiation time per pixel, 122 × 10-6 s; peak power per pixel (irradiation intensity), 3 × 105 W/cm2; overall dose per pixel (dose in mJ), 1.5 mJ/cm2.
UVA laser, 405 nm
Diode laser; laser power, 50 mW; irradiated area, 25.8 × 10-8 cm2; irradiation time, 4 s; resolution for image acquisition, 512 × 512; line average, 96; pixel size, 60.06 × 60.06 nm; image size, 30.74 × 30.74 μm; total number of irradiated pixels, 24,776; irradiation time per pixel, 161 × 10-6 s; peak power per pixel (irradiation intensity), 1.9 × 105 W/cm2; overall dose per pixel (radiation dose in mJ), 1.2 mJ/cm2.
Lamp from UVC Servis, Czech Republic; model GESP-15 15 W (UVA 330 to 400 nm wavelength, maximal efficiency 365 nm; geometry of irradiation, vertically downward; distance from the sample, 10 cm; irradiated area, 9.2 cm2 (cultivation plate area); irradiation time, 15 min.; dose, 0.828 J/cm2; cells were seeded at 6.5 × 104 cells/cm2 and irradiated 24 h after seeding without BrdU pre-sensitization.
Source, 60Co from Chisostat, Chirana, Prague; cells were cultivated on 22.1 cm2 cell cultivation plates at a density of 6.5 × 104 cells/cm2 and irradiated by 5 Gy of γ-rays (total dose); a maximum of four Petri dishes were irradiated on a rotation platform; the irradiation time was 2 to 3 min and the distance of the radiation source from the samples was 110 cm.
Immunofluorescence, Western blots, and immunoprecipitation
Cells on microscope slides were fixed in 4% formaldehyde for 10 min at room temperature, permeabilized sequentially in 0.2% Triton X-100 for 10 min and 0.1% saponin (Sigma-Aldrich, CZ) for 12 min, and washed twice in PBS for 15 min. Slides were blocked with 1% BSA dissolved in PBS for 1 h, washed for 15 min in PBS, and incubated with the following antibodies: anti-HP1β (#07-333, Upstate, USA or #MAB3448, Merck-Millipore, USA), anti-γH2AX (phospho S139; #ab2893, Abcam, UK), anti-53BP1 (#ab21083, Abcam); anti-UBF1 (H-300; sc-9131, Santa Cruz Biotechnology, USA), anti-RPA194 (#sc-48385, Santa Cruz Biotechnology), anti-TCOF (Treacher Collins syndrome protein, #ab65212, Abcam), and anti-fibrillarin (#ab5821, Abcam). Secondary antibodies used were: Alexa Fluor® 594 Donkey Anti-Mouse IgG (H + L) (A21203), Alexa Fluor® 594 Donkey Anti-Rabbit IgG (H + L) (A21207), Alexa Fluor® 488 Donkey Anti-Mouse IgG (H + L) (A21202), and Alexa Fluor® 488 Donkey Anti-Rabbit IgG (H + L) (A21206) (Molecular Probes®, Life Technologies, Czech Republic).
Western blotting was performed according to the method of Stixová et al.. For Western blot analysis of UBF1, we used anti-UBF1 (#sc9131, Santa Cruz), which detects UBF1 (97 kDa), UBF2 (94 kDa), and GFP-UBF1 (124 kDa). Apoptotic lamin B fragmentation was examined using anti-lamin B antibody (#sc-6217, Santa Cruz, USA) to determine whether GFP-UBF1 overexpression induced cell death. Anti-HP1β (HP1β: 28 kDa; GFP-HP1β: 55 kDa) (#MAB3448, Merck-Millipore) and anti-H3K9me3 (17 kDa) (#07-442, Merck-Millipore) antibodies were also used.
Immunoprecipitation was performed according to the method of Dawson et al.. We used the following antibodies for immunoprecipitation: anti-UBF1 (H-300; sc-9131, Santa Cruz Biotechnology, USA) (Figure 7A) and anti-HP1β (#MAB3448, Merck-Millipore) (Figure 7C). We performed immunoprecipitation with and without EtBr solution (E1510, Sigma-Aldrich, Czech Republic). To eliminate DNA-mediated protein interactions, EtBr was maintained in cell lysates at a concentration of 50 μg/ml during the entire immunoprecipitation process, including washing steps. We used 20 μg of total protein lysate as input. Immunoprecipitation was also performed with cells transfected by plasmid DNA encoding GFP-HP1β-ΔCSD and full-length GFP-HP1β. For Western blotting and immunoprecipitation analyses, we performed three biological replicates.
RNA interference (RNAi) for HP1β was performed using Lipofectamine RNAiMax transfection reagent (#13778-075, Invitrogen), siRNA (HP1β) (#sc-35588, Santa Cruz Biotechnology, Inc., Germany), control siRNA (#sc-36869, Santa Cruz Biotechnology, Inc.), and Opti-Mem reduced serum medium (#31985047, Invitrogen). The RNAi procedure was performed according to the manufacturer’s instructions for Lipofectamine RNAiMax reagent.
Analysis of GFP-UBF1 fluorescence intensity in nucleoli and non-nucleolar genomic regions after UVA irradiation
We selected three or four sub-regions with the same areas within irradiated and non-irradiated nucleolar regions to analyze GFP-UBF1 fluorescence intensity (Figure 3B,C). We analyzed fluorescence intensity in nucleoli and the nucleoplasm (that is, outside the nucleoli). The background fluorescence intensity (non-irradiated nucleoli or nucleoplasm) was subtracted from irradiated ROI fluorescence intensity. In some cases, this yielded negative values, owing to bleaching of the fluorochrome in irradiated regions, which reduced the level of fluorescence. This was observed primarily in nucleoli characterized by very slow UBF1 diffusion (; see Figure 3C for an example of negative values).
The FRET technique was performed using Leica SP5 X and Leica SPX8 confocal microscopes and the FRET mode of LEICA LAS AF software (version 2.1.2). To determine protein interactions, we used the FRET acceptor photobleaching technique. Proteins were labeled with mCherry and GFP (or by Alexa 594 and Alexa 488). Using these fluorochromes, FRET was optimized for well-known interacting partners, such as 53BP1 and p53, which yielded a FRET efficiency of approximately 40% (Figure 4A). To study protein interactions, we selected ROIs and measured donor fluorescence intensity before the bleaching step. We performed bleaching with 100% laser power for the acceptor. Finally, we measured fluorescence intensity after acceptor photobleaching, and calculated FRET efficiency using LEICA LAS AF software. We also analyzed UBF1 (visualized by Alexa 594) interaction with DNA stained by TO-PRO-3 iodide (642/661) (Life Technologies, Czech Republic).
RNA isolation and qRT-PCR
The fold change was calculated as:
The results are shown as fold change.
ataxia telangiectasia mutated
ataxia telangiectasia and Rad3-related protein
bovine serum albumin
cyclobutane pyrimidine dimer
chromo shadow domain
4',6-diamidino-2-phenylindole, DDR, DNA-damage response
Dulbecco’s modified Eagle’s medium
fetal bovine serum
Förster resonance energy transfer
fluorescent ubiquitination-based cell-cycle indicator
- GAPDH :
glyceraldehyde dehydrogenase gene
green fluorescence protein
heterochromatin protein 1
immortalized mouse embryonic fibroblasts
mouse embryonic fibroblasts
polymerase chain reaction
quantitative real-time polymerase chain reaction
red fluorescence protein
- RNA pol I:
RNA polymerase I
Region of interest
replication-related protein A
upstream binding factor 1
This work was supported by the Grant Agency of the Czech Republic, projects P302/10/1022, P302/12/G157, and 13-07822S. Partial support was also obtained from project 7F14369. The postdoctoral fellowships of LS and DVS were supported by the Education for Competitiveness Operational Programme (ECOP) CZ.1.07/2.3.00/30.0030. This work was also supported by program UNCE 204022 and Prvouk/ LF1/1 of Charles University. We thank BioScience Writers (Houston, TX, USA) for editing our revised manuscript. We also thank colleagues from the Brno University of Technology for assistance comparing FRET data obtained by the Leica TSC SP5 microscope with FRET data obtained by the Leica TSC SP8 confocal microscope.
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