Figure 4

DNA methylation protects regions of reversal of chromatin condensation upon suppression of H3K9me2. (A) Aggregate plots of FAIRE density at unmethylated promoter CGIs indicate that chromatin accessibility is completely reversed upon suppression of H3K9me2 for unmethylated promoter CGIs. (B) Scatter plot of FAIRE signals at unmethylated promoter CGIs demonstrates that changes in chromatin accessibility under UNC0638 treatment resemble changes at all H3K9me2 sites (Figure 1D). (C) Aggregate plots of FAIRE density at methylated promoter CGIs reveals a mitigated increase in chromatin accessibility upon loss of H3K9me2 at methylated promoter CGIs. (D) Scatter plot of FAIRE signals at methylated promoter CGIs confirms that methylated CGIs have less change in chromatin accessibility under UNC0638 treatment.