Volume 6 Supplement 1

Epigenetics and Chromatin: Interactions and processes

Open Access

Elucidating the mechanisms underlying GATA-1 activity

  • Roland Gamsjaeger1,
  • Ana Silva1,
  • Nicholas Shepherd1,
  • Lorna Wilkinson-White1,
  • Ernest Laue2,
  • Gerd Blobel3,
  • Jacgui Matthews1 and
  • Joel Mackav1
Epigenetics & Chromatin20136(Suppl 1):P49

DOI: 10.1186/1756-8935-6-S1-P49

Published: 18 March 2013

The fact that GATA-1 binds to gene regulatory elements that contain GATA sites, and thereby ultimately regulates the expression of hundreds of genes involved in hematopoietic development is well established. However, at a molecular level, the steps between DNA binding and changes in the expression levels of target genes are only partly understood, and the goal of our work is understand at a mechanistic level the changes in chromatin structure and occupancy and the protein interactions that are made to drive these changes in gene expression. We have recently shown that, surprisingly, the localization of GATA1 to its target sites in chromatin is dependent on acetylation of specific lysines in the protein, and have elucidated the structural basis for this requirement [1, 2]. Our data point towards a model for gene regulation in which not only are the post-translational modifications of histones essential for establishing gene expression patterns, but equivalent modifications in DNA-binding transcription factors are also an integral part of whatever ‘code’ underlies these patterns.

Concurrently, we have also determined the molecular details underlying the recruitment of the Nucleosome Remodeling and Deacetylase (NuRD) complex to GATA1-dependent gene promoters [3], an event that is essential for normal GATA1 activity. Combined with data that explore the molecular makeup of the NuRD complex, these results provide a glimpse into the mechanisms through which complex coregulator complexes are recruited to target genes and begin to map out the molecular events that drive gene regulation both in erythropoiesis and, by extension, in a range of other tissues.

Authors’ Affiliations

(1)
School of Molecular Bioscience, University of Sydney
(2)
Department of Biochemistry, University of Cambridge
(3)
Children’s Hospital of Philadelphia

References

  1. Lamonica et al:. PNAS. 2011, 108: 159-10.1073/pnas.1102140108.View ArticleGoogle Scholar
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  3. Lejon et al:. J Biol Chem. 2011, 286: 1196-10.1074/jbc.M110.195842.PubMed CentralView ArticlePubMedGoogle Scholar

Copyright

© Gamsjaeger et al; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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