Volume 6 Supplement 1

Epigenetics and Chromatin: Interactions and processes

Open Access

Methylation of histone H3 at lysine 23 in meiotic heterochromatin

  • Romeo Papazvan1,
  • Ekaterina Voronina2,
  • Jessica R Chapman3,
  • Tonya M Gilbert1,
  • Elizabeth Meier1,
  • Jeffrey Shabanowitz3,
  • Donald F Hunt3, 4,
  • Yifan Liu5 and
  • Sean D Taverna1
Contributed equally
Epigenetics & Chromatin20136(Suppl 1):O13

DOI: 10.1186/1756-8935-6-S1-O13

Published: 18 March 2013

Heterochromatin and its associated histone modifications are important for repressing transcription and maintaining chromosomal integrity during meiosis and mitosis. The complex repertoire of histone modifications that decorate heterochromatin has yet to be fully characterized, in part because most eukaryotic cells have a single nucleus where distinct chromatin types are intermingled on contiguous stretches of chromosomes. To obtain highly-purified heterochromatin, we turned to the model organism Tetrahymena thermophila, which has a biochemically separable heterochromatic micronucleus. We characterized combinatorial histone modifications on heterochromatic H3 from Tetrahymena and identified species of H3 dually-modified by both H3K23me3 and H3K27me3 as a previously unreported binary ‘mark’ specific for heterochromatin. Furthermore, H3K23me3 levels dramatically increased during meiosis in Tetrahymena micronuclei, C. elegans, and mice, suggesting this histone ‘mark’ plays a conserved role in germline development. Lastly, disrupting the H3K23 methyltransferase in Tetrahymena caused a lag in meiotic progression. Together, our data suggests H3K23me3 is a conserved heterochromatic histone PTM strongly associated with meiosis, and misregulation of this modification may be linked to problems with reproductive fitness and development.


Authors’ Affiliations

Department of Pharmacology and Molecular Sciences, and the Center for Epigenetics, The Johns Hopkins University School of Medicine
Department of Molecular Biology and Genetics and Howard Hughes Medical Institute, Center for Cell Dynamics, The Johns Hopkins University School of Medicine
Department of Chemistry, University of Virginia
Department of Pathology, University of Virginia
Department of Pathology, University of Michigan Medical School


© Papazvan et al; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.