Open Access

Erratum to: Multiple sequence-directed possibilities provide a pool of nucleosome position choices in different states of activity of a gene

  • Vinesh Vinayachandran1,
  • Rama-Haritha Pusarla1 and
  • Purnima Bhargava1Email author
Epigenetics & Chromatin20114:8

DOI: 10.1186/1756-8935-4-8

Received: 28 April 2011

Accepted: 7 May 2011

Published: 7 May 2011

The original article was published in Epigenetics & Chromatin 2009 2:4

Correction

After publication of this work [1] we noticed that there was on inadvertent oversight due to which Figure 1 (Figure 5) and 2 (Figure 6) Legends were interchanged. While Figures and their captions are correct, Legends should have been interchanged and read as follows.
Figure 5

Exo III mapping of the nucleosomes on the 5' Half SNR6 DNA fragments. Chromatin was assembled on the 248 bp DNA fragments labeled at the 5' end of either of the strands and digested by Exo III for different times. A 10 bp DNA ladder (Invitrogen) was end labeled and used as size marker in the lanes M. (A) Mapping on the top strand. Lanes 1 and 5 show uncut DNA; lanes 2-4 show naked DNA and 6-8 show chromatin. Sizes of the marker bands are given in the left hand side while arrows on the right hand side give the sizes of the DNA fragments due to the pauses of Exo III. (B) Schematic summary of mapping results from the panel A. Insert represents the cloned genomic DNA from -83 to +87 bp positions in the SNR6 gene while the flanking 43 and 34 bp are vector-derived DNA. The numbers on right hand site are the Exo III stops seen in the gel and represent the nucleosome boundaries. (C) Mapping on the bottom strand. Lanes 1 and 5 show uncut DNA; lanes 2-4 show naked DNA and 6-8 show chromatin. Sizes of the marker bands are given in the right hand side while arrows on the left hand side give the sizes of the DNA fragments due to the pauses of Exo III. (D) Schematic summary, similar to panel B, of the mapping results from the panel C. The numbers on right hand site are the Exo III stops seen in the gels and represent the nucleosome boundaries. (E) Schematic representation of mapping results on 5' half DNA from both the strands. Ovals represent nucleosomes while arrows show positions of their boundaries and Exo III stops, marked in upper portion of the cartoon. An asterisk in panels C, D and E shows the position of Exo III stop in the middle of the DNA fragment, at +2 bp position of SNR6.

Figure 6

Exo III mapping of the nucleosomes on the 3' Half SNR6 DNA fragments. Chromatin was assembled on the 272 bp DNA fragments labeled at the 5' end of either the top strand (panel A) or the bottom strand (panel B). A 10 bp DNA ladder (Invitrogen) was end labeled and used as size marker in the lanes M. Sizes of the marker bands are given in the left hand side of both the panels while arrows on the right hand side give the sizes of the DNA fragments due to the pauses of Exo III. Lanes 1 and 5 in both the panels show the uncut DNA. Rest of the lanes show naked DNA or chromatin digested for different times by Exo III. (C) Schematic representation of mapping results from the panels A and B. Insert represents the cloned genomic DNA from +62 to +256 bp positions in the SNR6 gene while the flanking 48 and 34 bp are vector-derived DNA. The numbers on right hand site are the Exo III pauses seen in the gels and represent the nucleosome boundaries.

Notes

Declarations

Authors’ Affiliations

(1)
Centre for Cellular & Molecular Biology (Council of Scientific and Industrial Research)

References

  1. Vinayachandran V, Haritha PR, Bhargava P: Multiple sequence-directed possibilities provide a pool of nucleosome position choices in different states of activity of a gene. Epigenetics and Chromatin. 2009, 2: 4.PubMed CentralView ArticlePubMedGoogle Scholar

Copyright

© Vinayachandran et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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