Figure 8From: The fungus Neurospora crassa displays telomeric silencing mediated by multiple sirtuins and by methylation of histone H3 lysine 9Expression of genes near telomeres is unaffected by nst mutations. Total RNA was extracted [47] from N3120 (nst+) and N3132 (nst-1RIP 1nst-3RIP 1nst-5RIP 1) and 20 μg was separated by agarose gel, blotted, and probed as described [48]. The 28S rRNA panel shows equivalent loading in both lanes by methylene blue staining of a blot. Other panels are autoradiograms after probing with the coding region of the gene closest to telomere IL (NCU010129.3), VIL (NCU07143.3), or VIIL (NCU09306.3).Back to article page