Figure 5From: The fungus Neurospora crassa displays telomeric silencing mediated by multiple sirtuins and by methylation of histone H3 lysine 9Histone H4 acetylation is increased in nst mutants. A) Western blots of nuclear extracts from strains N3120 (nst+) and N3132 (nst-1RIP 1nst-3RIP 1nst-5RIP 1) probed for the indicated epitopes. Different exposure times were used (not indicated) to avoid saturation of signal. B)In vitro NAD+-dependent deacetylase assays with the indicated H3 or H4 peptide substrates (NT, unmodified N-terminal peptide). Grey bars show activity for purified NST-1 protein; white bars represent GST (only) control.Back to article page