Figure 4From: The fungus Neurospora crassa displays telomeric silencing mediated by multiple sirtuins and by methylation of histone H3 lysine 9Derepression of the subtelomeric transgenes by inhibitors of histone acetyltransferases and DNA methyltransferases. A) Nicotinamide (NAM) relieved silencing of telomeric hph (N2285) but not hph flanked by amRIP(N2015) at a non-telomeric site. All plates contained 0.6 mg/ml Hygromycin. Plates with NAM contained 0.1 mg/ml (middle plate) or 1 mg/ml (right plate) of the drug. Each plate received approximately 100,000 conidia. B) Approximately 1000 conidia from an nst+ strain (N3120) carrying the telVR hph allele were plated in the presence or absence of 0.5 mg/ml Hygromycin to assay the effect of the drugs 5-azacytidine (5AC), Trichostatin A (TSA) and NAM on telomeric silencing. C) Same as B except strain N3441 carrying the tel VIIL bar allele was plated in the presence and absence of 4 mg/ml Basta. Control plates lacking Basta show a lawn of growth.Back to article page